Proteins and Proteomics: A Laboratory Manual

Simpson, Richard J.

Proteins and Proteomics: A Laboratory Manual - New Delhi I. K. International Publishing House Pvt. Ltd. 2003 - 926p

Preface: Acknowledgements: Foreword: Introduction to Proteomics One-dimensional Polyacrylamide Gel Electrophoresis Preparation of Cellular and Subcellular Extracts Preparative Two-dimensional Gel Electrophoresis with Immobilized pH Gradients Reversed-phase High-performance Liquid Chromatography Amino-and Carboxy-terminal Sequence Analysis Peptide Mapping and Sequence Analysis of Gel-resolved Proteins The Use of Mass Spectrometry in Proteomics Proteomic Methods for Phosphorylation Site Mapping Characterization of Protein Complexes Making Sense of Proteomics: Using Bioinformatics to Discover a Protein's Structure, Functions, and Interactions APPENDICES: Reference Tables Techniques Cautions Suppliers Index

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Proteins

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