Bioprospecting Microorganisms by Protoplast Fusion to Obtain Economically Important Products (Record no. 20602)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02133ngm a2200145Ia 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 140223b2009 xxu||||| |||| 00| 0 eng d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| Item number | DAV |
| 245 ## - TITLE STATEMENT | |
| Title | Bioprospecting Microorganisms by Protoplast Fusion to Obtain Economically Important Products |
| Statement of responsibility, etc | by Jui Dave, Pankti Jain and Shanmukhi Rao |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Ahmedabad |
| Name of publisher, distributor, etc | Institute of Science, Nirma University |
| Date of publication, distribution, etc | 2009 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 61p |
| 500 ## - GENERAL NOTE | |
| General note | The aim of the present investigation is to obtain superior strains with increased activity of industrial enzymes like glucose isomerase (GI), rennin like enzyme and chitinase through protoplast fusion. Protoplasts were obtained by using lysozyme and fusion was carried out using polyethylene glycol prepared in STC buffer. The protoplasts were confirmed by cell wall staining (Dyar’s method) and screened by growth on selective media containing combination of specific antibiotics. As Streptomyces griseus, S. griseoruber and S. olivaceus are potent producers of GI, they were selected as parental strains for fusion. Three fusant strains were obtained, namely, GIf1 from fusion of S. olivaceus and S. griseus, GIf2 from S. olivaceus and S. griseoruber and GIf3 from S. griseus and S. griseoruber. Among the three fusants, the strain GIf3 showed maximum GI activity which was 1.5 times more than the parent strains. Rennin like enzyme is extensively used in dairy industry to produce cheese from milk. Among the three fusants, namely, Rf1, Rf2 and Rf3, which were all obtained by fusion of Lactococcus lactis and Lactobacillus planatarum, the fusants Rf1 and Rf3 produced 1.3 times more rennin like enzyme than the parent strains. Chitinase, which catalyses the breakdown of chitin, finds immense application in agriculture and pesticide industry. Strains with higher chitinase activity were to be obtained by self fusion of Trichoderma harzianum. However this part of the work was not carried out due to lack of lysing enzyme, Novzym 234, used for removal of fungal cell wall. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dave, Jui; Rao, Shanmukhi and Jain, Pankti. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Dissertation |
| 995 ## - RECOMMENDATION 995 [LOCAL, UNIMARC FRANCE] | |
| Origin of item (home branch) (free text) | Science Dissertation |
| Genre | SDR00072 |
| Section | 660.6 |
| Recipient parent organisation code | SDR00072 |
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