Study of in vitro frequency of micronuclei in short-term cultured blood lymphocytes in subjects with long term exposure to tobacco (Record no. 20758)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 01797ngm a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20170531155821.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 140223b2011 xxu||||| |||| 00| 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| Terms of availability | 0.00 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572 |
| Item number | VAR |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Varghese, Jinitha |
| 9 (RLIN) | 13449 |
| 245 ## - TITLE STATEMENT | |
| Title | Study of in vitro frequency of micronuclei in short-term cultured blood lymphocytes in subjects with long term exposure to tobacco |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Ahmedabad |
| Name of publisher, distributor, etc. | Institute of Science, Nirma University |
| Date of publication, distribution, etc. | 2011 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 43p |
| 500 ## - GENERAL NOTE | |
| General note | 09MMB020 ABSTRACT:- Cytokinesis Blocked Micronucleus (CBMN) assay is widely used biomarker for measuring anomalies like chromosome breakage and chromosome missegregation. Chromosome breakage can occur due to exposure to various carcinogens including chemicals, drugs, alcohol and environmental factors. Micronucleus frequency is also influenced by the age, diet and gender. Tobacco smoking is a significant source of exposure to a large number of substances known to be hazardous to human health. Smoking causes DNA single strand breaks, DNA adducts and oxidative damage to DNA. Here whole blood samples were taken from smokers (10-20 cigarettes/day) and non smokers. Samples were cultured and allowed to complete one cell cycle and were arrested at the cytokinesis stage by using Cytochalasin –B which gave the binucleated cells. 1000 binucleated cells were scored to see the presence of micronucleus. Mitotic index of each sample was also calculated which depends on each individuals response to PHA (a mitogen stimulating agent). |
| 600 ## - SUBJECT ADDED ENTRY--PERSONAL NAME | |
| Personal name | Biochemistry |
| 9 (RLIN) | 13450 |
| 600 ## - SUBJECT ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cytochalasin-B |
| 9 (RLIN) | 13451 |
| 600 ## - SUBJECT ADDED ENTRY--PERSONAL NAME | |
| Personal name | Micronucleus |
| 9 (RLIN) | 13452 |
| 600 ## - SUBJECT ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tobacco |
| 9 (RLIN) | 13453 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Dissertation |
No items available.