Condensed Protocols- From Molecular Cloning: A Laboratory Manual

Plasmids and their usefulness in molecular cloning Bacteriophage and its vectors Working with bacteriophage M13 vectors Working with high-capacity vectors Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis Preparation and analysis of eukaryotic genomic DNA Extraction, purification, and analysis of mRNA from eukaryotic cells In vitro amplification of DNA by the polymerase chain reaction Preparation of radio labeled DNA and RNA probes Working with synthetic oligonucleotide probes Preparation of cDNA libraries and gene identification DNA sequencing Mutagenesis Screening expression libraries Expression of cloned genes in Escherichia coli Introducing cloned genes into cultured mammalian cells Analysis of gene expression in cultured mammalian cells Protein interaction technologies.