Molecular Biology Lab Fax Vol. II: Gene Analysis

Abbreviations. Nucleic Acid Blotting and Hybridization: Membranes for Nucleic Acid Blotting. Factors Influencing Hybridization. Synthetic Oligonucleotide Probes. Reagents and Conditions for Nucleic Acid Hybridization. DNA Sequencing: Chain Termination Sequencing. Chemical Degradation Sequencing. Denaturing Polyacrylamide Gel Electrophoresis. Troubleshooting. DNA Databases and Software for Sequence Analysis. Polymerase Chain Reaction: Amplification Methodology. Cloning PCR Products. Sequencing PCR Products. Troubleshooting. Labeling Nucleic Acids: Radiochemistry. Labeling Procedures. Detection of Radioactivity. Protection from Radiochemical Hazards. Non-Radioactive Labeling. Agarose Gel Electrophoresis: Agarose Gels. Electrophoresis Systems. DNA and RNA Markers for Agarose Gels. Centrifugation: Calculation of the Relative Centrifugal Force. Density Gradient Centrifugation. Care of Rotors. Chemicals and Reagents: Details of Individual Compounds. Safety: Protection from Chemical Hazards. Protection from Microbiological Hazards. Chapter References. Subject Index.