Practical HPLC Method Development

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations. critical band pair, benzoic acid sample, initial gradient run, ionic samples, isocratic method development, dwell volume, changing solvent type, gradient elution method development, late eluters, initial separation conditions, separating basic compounds, overloaded separation, changing column type, method ruggedness, reagent uptake, silanol effects, column killers, column plate number, retention range, herbicide sample, cyano column, method development proceeds, oligo samples, peak tracking, starting mobile phase