000			01488nam a2200193Ia 4500
008			140223b2006 xxu||||| |||| 00| 0 eng d
020			_a9780879698126 _c0.00
082			_a572.8 _bSAM
100			_aSambrook, Joseph _9111858
245			_aCondensed Protocols- From Molecular Cloning: A Laboratory Manual
260			_aNew York _bCold Spring Harbor Laboratory Press _c2006
300			_a800p
500			_aPlasmids and their usefulness in molecular cloning Bacteriophage and its vectors Working with bacteriophage M13 vectors Working with high-capacity vectors Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis Preparation and analysis of eukaryotic genomic DNA Extraction, purification, and analysis of mRNA from eukaryotic cells In vitro amplification of DNA by the polymerase chain reaction Preparation of radio labeled DNA and RNA probes Working with synthetic oligonucleotide probes Preparation of cDNA libraries and gene identification DNA sequencing Mutagenesis Screening expression libraries Expression of cloned genes in Escherichia coli Introducing cloned genes into cultured mammalian cells Analysis of gene expression in cultured mammalian cells Protein interaction technologies.
600			_aMolecular Cloning _9111859
700			_aRussell, David W. _9111860
942			_2ddc _k572.8 _mSAM _cREF
995			_aScience Reference _eS0000435 _j572.8 _yS0000435
995			_aScience Lending _eS0000434 _j572.8 _yS0000434
999			_c19012 _d19012