000			01797ngm a2200241Ia 4500
003			OSt
005			20170531155821.0
008			140223b2011 xxu||||| |||| 00| 0 eng d
020			_c0.00
040			_c
082			_a572 _bVAR
100			_aVarghese, Jinitha _913449
245			_aStudy of in vitro frequency of micronuclei in short-term cultured blood lymphocytes in subjects with long term exposure to tobacco
260			_aAhmedabad _bInstitute of Science, Nirma University _c2011
300			_a43p
500			_a09MMB020 ABSTRACT:- Cytokinesis Blocked Micronucleus (CBMN) assay is widely used biomarker for measuring anomalies like chromosome breakage and chromosome missegregation. Chromosome breakage can occur due to exposure to various carcinogens including chemicals, drugs, alcohol and environmental factors. Micronucleus frequency is also influenced by the age, diet and gender. Tobacco smoking is a significant source of exposure to a large number of substances known to be hazardous to human health. Smoking causes DNA single strand breaks, DNA adducts and oxidative damage to DNA. Here whole blood samples were taken from smokers (10-20 cigarettes/day) and non smokers. Samples were cultured and allowed to complete one cell cycle and were arrested at the cytokinesis stage by using Cytochalasin –B which gave the binucleated cells. 1000 binucleated cells were scored to see the presence of micronucleus. Mitotic index of each sample was also calculated which depends on each individuals response to PHA (a mitogen stimulating agent).
600			_aBiochemistry _913450
600			_aCytochalasin-B _913451
600			_aMicronucleus _913452
600			_aTobacco _913453
942			_2ddc _cDIS
999			_c20758 _d20758